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Biochemistry & Microbiology

Molecular biology of living cells, metabolism, nucleic acids, immunology, microorganisms, sterilization, and microbial assay.

10 Units120 Total Marks180 Teaching Hours

Units

1

[Bio] Introduction to Biochemistry

Importance of biochemistry for health science.

2 hrs2 marks

Subtopics (2)

  • Introduction to biochemistry
  • Importance for health science
2

[Bio] Biomolecules

Carbohydrates, amino acids, lipids, vitamins, and enzymes — metabolism and clinical significance.

26 hrs20 marks

Subtopics (6)

  • Carbohydrates: Glycolysis, Citric acid cycle
  • Amino acids, Peptides and Proteins: Urea cycle
  • Lipids and fatty acids: Beta-oxidation
  • Relation of Carbohydrate, Fat and Protein metabolism
  • Vitamins
  • Enzymes: Coenzymes, Isoenzymes
3

[Bio] Nucleic Acids & rDNA Technology

DNA, RNA, replication, transcription, translation, recombinant DNA technology, PCR.

20 hrs15 marks

Subtopics (5)

  • Nucleotides, nucleosides, nucleic acids, genes, chromosomes
  • DNA vs RNA
  • DNA replication, transcription, translation
  • Recombinant DNA technology and pharmaceutical products
  • Gene sequencing and PCR
4

[Bio] Laboratory & Diagnostic Tests

Common lab tests: CBC, LFT, KFT, thyroid, lipid profile, blood sugar, urine/stool analysis.

6 hrs5 marks

Subtopics (6)

  • Complete Blood Count (CBC)
  • Liver Function Test (LFT)
  • Kidney Function Test (KFT)
  • Thyroid Function Test
  • Lipid Profile and Blood Sugar
  • Urine and Stool analysis
5

[Bio] Fundamentals of Immunology

Immunity, antigens, antibodies, vaccines, T and B lymphocytes.

6 hrs5 marks

Subtopics (5)

  • Immunity and its types
  • Antigens, Antibodies, Immunoglobulins
  • Vaccines, Sera and Antisera
  • Types of vaccines with examples
  • T and B-lymphocytes comparison
6

[Micro] Introduction to Microbiology

Definition, importance, branches, and history of pharmaceutical microbiology.

5 hrs5 marks

Subtopics (4)

  • Definition and importance of microbiology
  • Branches of microbiology
  • Historical development of pharmaceutical microbiology
  • Applications in pharmaceutical sciences
7

[Micro] Microorganisms

Bacteria (morphology, gram staining, growth curve), viruses, and fungi.

25 hrs15 marks

Subtopics (9)

  • Bacterial morphology and classification
  • Gram positive vs Gram negative bacteria
  • Bacterial growth curve
  • Nutritional requirements and growth factors
  • Culture media types and preparation
  • Staining techniques (Simple, Gram, AFB staining)
  • Bacterial resistance mechanisms
  • Virus: definition, classification, morphology
  • Fungi/Yeasts/Molds: definition, morphology, pharmaceutical importance
8

[Micro] Normal Flora

Normal flora of skin, GIT, ear, nose, and genitourinary tract.

4 hrs3 marks

Subtopics (4)

  • Normal flora of skin
  • Normal flora of gastrointestinal tract
  • Normal flora of ear, nose, and genitourinary tract
  • Roles in normal physiology
9

[Micro] Sterilization

Methods of sterilization and disinfection, sterility testing.

20 hrs15 marks

Subtopics (4)

  • Definition of sterilization and disinfection
  • Methods of sterilization with examples
  • Methods of disinfection with examples
  • Sterility testing: Membrane filtration method and LAL test
10

[Micro] Microbiological Assay

Microbial assay of antibiotics and vitamins.

6 hrs5 marks

Subtopics (2)

  • Microbial assay of antibiotics (disc diffusion and tube dilution)
  • Microbial assay of vitamins (cyanocobalamine)